654 research outputs found
Tuning and Switching a Plasmonic Quantum Dot Sandwich in a Nematic Line Defect
We study the quantum-mechanical effects arising in a single semiconductor
core/shell quantum dot controllably sandwiched between two plasmonic nanorods.
Control over the position and the sandwich confinement structure is achieved by
the use of a linear-trap, liquid-crystal line defect and laser tweezers that
push the sandwich together. This arrangement allows for the study of exciton
plasmon interactions in a single structure, unaltered by ensemble effects or
the complexity of dielectric interfaces. We demonstrate the effect of plasmonic
confinement on the photon-antibunching behavior of the quantum dot and its
luminescence lifetime. The quantum dot behaves as a single emitter when
nanorods are far away from the quantum dot but shows possible multiexciton
emission and a significantly decreased lifetime when tightly confined in a
plasmonic sandwich. These findings demonstrate that liquid crystal defects,
combined with laser tweezers, enable a versatile platform to study plasmonic
coupling phenomena in a nanoscale laboratory, where all elements can be
arranged almost at will.Comment: Supporting information at the en
Composite Skyrmion bags in two-dimensional materials
Skyrmions are particle-like topological excitations, studied in various
condensed matter systems and models of high-energy physics (HEP). They occur as
stable spin textures in certain planar magnetic materials and as configurations
in chiral nematic liquid crystals, having been originally proposed as model of
atomic nuclei. Since magnetic Skyrmions can be accelerated with a current, they
have the potential to encode bits in low-power magnetic storage devices.
Drawing on techniques from HEP, we demonstrate that magnetic and liquid crystal
Skyrmions interact like orientation dependent, localised particles, explaining
previously observed Skyrmion behaviour. This interaction motivates the
construction of Skyrmion bags: textures of high topological degree which we
realise experimentally in liquid crystals, and in magnetic materials by
computer simulations. These Skyrmion bags configurations are nested multiple
Skyrmions, which act like single Skyrmions in pairwise interaction, and under
the influence of a current in magnetic materials. These results emphasize
equivalent Skyrmion behaviour in different physical systems, and suggest new,
high-density magnetic memory storage devices based on Skyrmion bags.Comment: paper including methods and SI; 7 figures, 14 page
Electromechanical reciprocity and arrhythmogenesis in long-QT syndrome and beyond.
An abundance of literature describes physiological and pathological determinants of cardiac performance, building on the principles of excitation-contraction coupling. However, the mutual influencing of excitation-contraction and mechano-electrical feedback in the beating heart, here designated 'electromechanical reciprocity', remains poorly recognized clinically, despite the awareness that external and cardiac-internal mechanical stimuli can trigger electrical responses and arrhythmia. This review focuses on electromechanical reciprocity in the long-QT syndrome (LQTS), historically considered a purely electrical disease, but now appreciated as paradigmatic for the understanding of mechano-electrical contributions to arrhythmogenesis in this and other cardiac conditions. Electromechanical dispersion in LQTS is characterized by heterogeneously prolonged ventricular repolarization, besides altered contraction duration and relaxation. Mechanical alterations may deviate from what would be expected from global and regional repolarization abnormalities. Pathological repolarization prolongation outlasts mechanical systole in patients with LQTS, yielding a negative electromechanical window (EMW), which is most pronounced in symptomatic patients. The electromechanical window is a superior and independent arrhythmia-risk predictor compared with the heart rate-corrected QT. A negative EMW implies that the ventricle is deformed-by volume loading during the rapid filling phase-when repolarization is still ongoing. This creates a 'sensitized' electromechanical substrate, in which inadvertent electrical or mechanical stimuli such as local after-depolarizations, after-contractions, or dyssynchrony can trigger abnormal impulses. Increased sympathetic-nerve activity and pause-dependent potentiation further exaggerate electromechanical heterogeneities, promoting arrhythmogenesis. Unraveling electromechanical reciprocity advances the understanding of arrhythmia formation in various conditions. Real-time image integration of cardiac electrophysiology and mechanics offers new opportunities to address challenges in arrhythmia management
Feminae: an international multi-site innovative project for female athletes.
Sufficient high-quality studies in sport science using women as participants are lacking, meaning that our knowledge and understanding of female athletes in relation to their ovarian hormone profiles is limited. Consortia can be used to pool talent, expertise, and data, thus accelerating our learning on a given topic and reducing research waste through collaboration. To this end, we have assembled an international multi-site team, described herein, to investigate the effects of the menstrual cycle and oral contraceptive pill phase on aspects of exercise physiology and sports performance in female athletes. We intend to produce an adequately powered, high-quality dataset which can be used to inform the practices of female athletes. Our approach will also employ research transparency – through the inclusion of a process evaluation - and reproducibility – through a standardised study protocol
A novel targeted/untargeted GC-Orbitrap metabolomics methodology applied to Candida albicans and Staphylococcus aureus biofilms
Introduction:
Combined infections from Candida albicans and Staphylococcus aureus are a leading cause of death in the developed world. Evidence suggests that Candida enhances the virulence of Staphylococcus—hyphae penetrate through tissue barriers, while S. aureus tightly associates with the hyphae to obtain entry to the host organism. Indeed, in a biofilm state, C. albicans enhances the antimicrobial resistance characteristics of S. aureus. The association of these microorganisms is also associated with significantly increased morbidity and mortality. Due to this tight association we hypothesised that metabolic effects were also in evidence.
Objectives:
To explore the interaction, we used a novel GC-Orbitrap-based mass spectrometer, the Q Exactive GC, which combines the high peak capacity and chromatographic resolution of gas chromatography with the sub-ppm mass accuracy of an Orbitrap system. This allows the capability to leverage the widely available electron ionisation libraries for untargeted applications, along with expanding accurate mass libraries and targeted matches based around authentic standards.
Methods:
Optimised C. albicans and S. aureus mono- and co-cultured biofilms were analysed using the new instrument in addition to the fresh and spent bacterial growth media.
Results:
The targeted analysis experiment was based around 36 sugars and sugar phosphates, 22 amino acids and five organic acids. Untargeted analysis resulted in the detection of 465 features from fresh and spent medium and 405 from biofilm samples. Three significantly changing compounds that matched to high scoring library fragment patterns were chosen for validation.
Conclusion:
Evaluation of the results demonstrates that the Q Exactive GC is suitable for metabolomics analysis using a targeted/untargeted methodology. Many of the results were as expected: e.g. rapid consumption of glucose and fructose from the medium regardless of the cell type. Modulation of sugar-phosphate levels also suggest that the pentose phosphate pathway could be enhanced in the cells from co-cultured biofilms. Untargeted metabolomics results suggested significant production of cell-wall biosynthesis components and the consumption of non-proteinaceous amino-acids
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